Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
AR

Cell type

Cell type Class
Prostate
Cell type
LAPC-4
NA
NA

Attributes by original data submitter

Sample

source_name
LAPC4 (prostate)
chip antibody
AR
cell line
LAPC4
treatment
Darolutamide

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For chromatin immunoprecipitation experiments with subsequent sequencing (ChIP-seq), three replicates of eight million cells were seeded in 15-cm plates and treated with 2 µM darolutamide plus 1 nM R1881 for 22 h, subsequently fixed for ten minutes with 1% formaldehyde and processed as described previously (Baumgart et al. Nucleic Acids Res. 2017 Jul 27;45(13):7722-7735) Library preparation was done as described by the manufacturer (MicroPlex Library Preparation Kit v2; Diagenode SA, Seraing, Belgium). The libraries were sequenced on a HiSeq2500 Illumina machine with 50 base pair, single-end reads to an average depth of 25–30 million reads per sample.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
27927749
Reads aligned (%)
79.1
Duplicates removed (%)
19.1
Number of peaks
934 (qval < 1E-05)

hg38

Number of total reads
27927749
Reads aligned (%)
81.0
Duplicates removed (%)
17.7
Number of peaks
771 (qval < 1E-05)

Base call quality data from DBCLS SRA