GSM4462692: BRD4 VCaP R1881 Rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
TFs and others
Cell type Class
Attributes by original data submitter
Sequenced DNA Library
For chromatin immunoprecipitation experiments with subsequent sequencing (ChIP-seq), three replicates of eight million cells were seeded in 15-cm plates and treated with 2 µM darolutamide plus 1 nM R1881 for 22 h, subsequently fixed for ten minutes with 1% formaldehyde and processed as described previously (Baumgart et al. Nucleic Acids Res. 2017 Jul 27;45(13):7722-7735) Library preparation was done as described by the manufacturer (MicroPlex Library Preparation Kit v2; Diagenode SA, Seraing, Belgium). The libraries were sequenced on a HiSeq2500 Illumina machine with 50 base pair, single-end reads to an average depth of 25–30 million reads per sample.