GSM4459503: T47D input ChIPseq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Breast
Cell type
Mammary gland ductal carcinoma
NA
NA
Attributes by original data submitter
Sample
source_name
mammary gland ductal carcinoma
parental cell line
T-47D
esr1 genotype
WT/Y537S/D538G
chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Fixation was performed by treating cells with 1% formaldehyde for 10 minutes at room temperature. The cross-linking reaction was stopped with the addition of glycine to a final concentration of 125 mM. Cells were then washed with cold PBS and harvested via cell scraping in Farnham lysis buffer supplemented with protease inhibitors. Chromatin immunoprecipitation was performed as previously described (Reddy et al. Genome Research 2009) with either Anti-FLAG (Sigma-Aldrich M2) antibody that recognizes a FLAG tag on ESR1, Anti-CTCF (sc-5916, Santa Cruz Biotechnology), or Anti-OCT1 (A301-716A and A301-717A, Bethyl Laboratories Inc.) antibodies.