Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Epidermis

Cell type

501A

Primary Tissue

Skin

Tissue Diagnosis

Melanoma

Sample

source_name

501Mel

cell line

501Mel

cell type

melanoma

chip antibody

H3K4me3 : Millipore (04-745)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP and ChIP-seq experiments were performed on chromatin from native 501Mel cells or the clone 8 cells according to standard protocols as previously described (Delacroix et al., 2010; Kobi et al., 2010). The libraries were sequenced on Illumina GAIIx sequencer (Illumina, San Diego, CA, USA) as single-end 36 base reads following Illumina’s instructions

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

hg38

Number of total reads

11192622

Reads aligned (%)

86.2

Duplicates removed (%)

49.2

Number of peaks

22515 (qval < 1E-05)

hg19

Number of total reads

11192622

Reads aligned (%)

85.9

Duplicates removed (%)

49.5

Number of peaks

22528 (qval < 1E-05)