GSM4455704: GM04604 CTCF IP; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
CTCF
Cell type
Cell type Class
Blood
Cell type
GM04604
NA
NA
Attributes by original data submitter
Sample
source_name
GM04604 (UN-B)
biomaterial_provider
GM04604
cell type
Lymphoblastoid Cell Line
disease
Unaffected
chip antibody
Diagenode, C15410210
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation was performed according to the Diagenode AutoiDeal ChIP-qPCR kit (Diagenode) standard protocol. Samples of 6 x 106 cells were sonicated using the Diagenode Bioruptor Pico (Diagenode), with 10 cycles of 30 sec “on” and 30 sec “off”. Correct DNA fragmentation was verified by agarose gel electrophoresis. Immunoprecipitation was performed with 4 x 10^6 cells using a CTCF antibody (Diagenode). CTCF ChIP-seq libraries were prepared in triplicate using the Illumina TruSeq ChIP Sample Preparation kit. We performed 1 x 50 bp single-end sequencing with Illumina Hiseq 4000.