Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Kidney
Cell type
Kidney Cortex
MeSH Description
The outer zone of the KIDNEY, beneath the capsule, consisting of KIDNEY GLOMERULUS; KIDNEY TUBULES, DISTAL; and KIDNEY TUBULES, PROXIMAL.

Attributes by original data submitter

Sample

source_name
Mouse kidney cortex, input
strain/background
CD-1
genotype/variation
wildtype
tissue
kidney cortex
age
3 weeks
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq: Kidney cortex was dissected and crosslinked with formaldehyde. Chromatin was extracted, cleared and WT1-DNA complexes were enriched using a WT1 antibody (C-19; Santa Cruz, sc-192, Lot F1711). Libraries for sequencing were prepared using standard Illumina protocols.

Sequencing Platform

instrument_model
Illumina Genome Analyzer

mm10

Number of total reads
31376693
Reads aligned (%)
95.0
Duplicates removed (%)
14.3
Number of peaks
522 (qval < 1E-05)

mm9

Number of total reads
31376693
Reads aligned (%)
94.8
Duplicates removed (%)
14.3
Number of peaks
587 (qval < 1E-05)

Base call quality data from DBCLS SRA