Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
HEK293 CDK12as
cell line
HEK293 CDK12as
antibody
none
treatment
DMSO

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA were purified from chromatin IP products. Total RNA were purified from immunoprecipitated RNA polymerase II. For ChIP-seq, libraries were prepared using Illumina's TruSeq ChIP library Preparation Kit according to Illumina's instructions. For mNET-seq, libraries were prepared using NEBNext® Small RNA Library Prep Set for Illumina, according to NEB's instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg38

Number of total reads
37602136
Reads aligned (%)
96.8
Duplicates removed (%)
9.9
Number of peaks
922 (qval < 1E-05)

hg19

Number of total reads
37602136
Reads aligned (%)
95.8
Duplicates removed (%)
10.1
Number of peaks
487 (qval < 1E-05)

Base call quality data from DBCLS SRA