Antigen

Antigen Class

TFs and others

Antigen

CPSF3

Cell type

Cell type Class

Kidney

Cell type

293

Primary Tissue

Kidney

Tissue Diagnosis

Normal

Sample

source_name

HEK293 CDK12as

cell line

HEK293 CDK12as

antibody

A301-091A, Bethyl Laboratories

treatment

DMSO

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

DNA were purified from chromatin IP products. Total RNA were purified from immunoprecipitated RNA polymerase II. For ChIP-seq, libraries were prepared using Illumina's TruSeq ChIP library Preparation Kit according to Illumina's instructions. For mNET-seq, libraries were prepared using NEBNext® Small RNA Library Prep Set for Illumina, according to NEB's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg19

Number of total reads

72740952

Reads aligned (%)

60.3

Duplicates removed (%)

57.4

Number of peaks

312 (qval < 1E-05)

hg38

Number of total reads

72740952

Reads aligned (%)

60.9

Duplicates removed (%)

56.9

Number of peaks

403 (qval < 1E-05)