Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Digestive tract

Cell type

Gastric epithelial cells

NA

NA

Sample

source_name

Normal gastric epithelial cell

cell type

uninfected control cell

chip antibody

H3K27ac_ChIP (Active Motif, 91193, lot 01518001)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-seq, sonicated nuclear fraction was incubated with each antibody and histone-DNA complexes were isolated. ChIP-seq libraries were prepared using KAPA Hyper Prep Kit (KAPA Biosystems) following the manufacturer's protocol.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

23079230

Reads aligned (%)

98.5

Duplicates removed (%)

7.3

Number of peaks

32210 (qval < 1E-05)

hg19

Number of total reads

23079230

Reads aligned (%)

98.0

Duplicates removed (%)

8.2

Number of peaks

32138 (qval < 1E-05)