Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Adult
Cell type
Germline cells
NA
NA

Attributes by original data submitter

Sample

source_name
Isolated germ nuclei from oef-1(vr25) mutant adults
Stage
Young adult
tissue
Isolated germ nuclei
antibody
H3K27me3
strain
YL585
genotype
oef-1(vr25)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Isolated germ cell nuclei were sonicated in a Misonix S-4000 water bath sonicator, and immunoprecipated overnight using 5 µg of anti-H3K27me3 or anti-H3K36me3 antibody. ChIP-seq library preparation for enriched DNA and the genomic DNA input control for two biological replicates per genotype was performed as described in (Zhong et al. 2010). Libraries were multiplexed as described in Lefrancois et al. (2009) in order to sequence four samples in a single flow cell using the Illumina Genome Analyzer II platform.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

ce11

Number of total reads
21768229
Reads aligned (%)
96.3
Duplicates removed (%)
18.2
Number of peaks
1708 (qval < 1E-05)

ce10

Number of total reads
21768229
Reads aligned (%)
96.3
Duplicates removed (%)
18.2
Number of peaks
1706 (qval < 1E-05)

Base call quality data from DBCLS SRA