Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Embryonic fibroblast
Cell type
MEF
Tissue
Embryonic Fibroblast
Lineage
primaryCells
Description
Mouse Embryonic Fibroblast

Attributes by original data submitter

Sample

source_name
Mouse Embryonic Fibroblast (MEF)
cells
Mouse Embryonic Fibroblast (MEF)
embryo stage
E15.5
passages
2_to_4
strain
C57BL/6
genotype/variation
Hmga2 Ko
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated chromatin and Protein-DNA complexes were isolated with the antibody. Libraries were prepared according to Illumina's instructions accompanying the Ovation Ultra Low Kit

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
45269705
Reads aligned (%)
97.3
Duplicates removed (%)
13.6
Number of peaks
326 (qval < 1E-05)

mm9

Number of total reads
45269705
Reads aligned (%)
97.1
Duplicates removed (%)
13.6
Number of peaks
289 (qval < 1E-05)

Base call quality data from DBCLS SRA