Curated Sample Data


Genome
ce10
Antigen Class
Input control
Antigen
Input control
Cell type Class
Adult
Cell type
Adult

Cell type information


NA
NA

Attributes by Original Data Submitter


source_name
whole worm lysate
background genotype
Wildtype
treated with
spg-7(RNAi)
chip antibody
No Antibody

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cross-linking of DNA and protein was performed by treating the worms with 11% formaldehyde at 4°C for one hour. The worms were then lysed via Teflon homogenizer and sonicated to obtain 500-1000 base pair DNA fragments. ATFS-1 was then immunopreciptated from 500 mg of lysate using ATFS-1 antibodies [PMID 22700657] and protein A sepharose beads (Invitrogen). The cross-links were reversed by incubation at 65°C for 5 hours. The DNA fragments were then ethanol-precipitated and purified using QiaQuick Spin Columns (Qiagen). The DNA fragments were sequenced using the Hi-Seq Illumina platform.

Platform Information


instrument_model
Illumina HiSeq 2500

External Database Query

Logs in read processing pipeline


Number of total reads
27099673
Reads aligned (%)
0.2
Duplicates removed (%)
17.0
Number of peaks
120 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA