GSM1557621: Wt spg-7 with antibody; Caenorhabditis elegans; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
atfs-1
Cell type
Cell type Class
Adult
Cell type
Whole worm
NA
NA
Attributes by original data submitter
Sample
source_name
whole worm lysate
background genotype
Wildtype
treated with
spg-7(RNAi)
chip antibody
ATFS-1 antibody
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cross-linking of DNA and protein was performed by treating the worms with 11% formaldehyde at 4°C for one hour. The worms were then lysed via Teflon homogenizer and sonicated to obtain 500-1000 base pair DNA fragments. ATFS-1 was then immunopreciptated from 500 mg of lysate using ATFS-1 antibodies [PMID 22700657] and protein A sepharose beads (Invitrogen). The cross-links were reversed by incubation at 65°C for 5 hours. The DNA fragments were then ethanol-precipitated and purified using QiaQuick Spin Columns (Qiagen). The DNA fragments were sequenced using the Hi-Seq Illumina platform.