Diffuse large B-cell lymphoma activated B-cell type
Sequenced DNA Library
Exponentially growing cells were cross-linked with 1% formaldehyde and then quenched with glycine for 5 min at RT followed by lysis and MNase digestion. Samples were sonicated for 60 min using the Bioruptor (Diagenode) For each immunoprecipitation reaction, 4x106 chromatin cell equivalent were incubated overnight with either an AID antibody (Abcam, ab59361) or a rabbit IgG negative control antibody, subjected to protein A/G magnetic bead binding, IP elution and DNA recovery. The ChIP DNA was used to generate ChIPseq libraries with the ChIP-Seq sample preparation kit (Illumina) for sequencing at the Arkansas Children's Research Institute Genomics Core.