GSM4403406: PD-L1 Rep2 Input; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Breast
Cell type
MDA-MB-231
Primary Tissue
Breast
Site of Extraction
Effusion, Pleural
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
MDA-MB-231
genotype
wild type
cell line
MDA-MB-231
tumor type
Triple-negative breast cancer
chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and DNA complexes were isolated with HA antibody. Approximately 2-5 ng of ChIPed DNA from each condition was used to generate the ChIP-seq library using the Ovation Ultralow DR Multiplex System 1-8 Kit (NuGEN Technologies), according to the manufacturer's protocol.