GSM4395566: MDA-MB-231 3f:S JQ1 neg ChIP S; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
BRD4
Cell type
Cell type Class
Breast
Cell type
MDA-MB-231
Primary Tissue
Breast
Site of Extraction
Effusion, Pleural
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
MDA-MB-231 3F:BRD4-S(a)
cell line
breast cancer cell line MDA-MB-231 ectopic 3f:BRD4-S(a) expression
er/pr/her2 statue
triple negative – ER-; PR-; HER2-
chip antibody
anti-BRD4-S(a)
bet inhibitor jq1 treatment
inactive enantiomer JQ1, i.e. JQ1_neg or JQ1(-)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated by IP with anti-BRD4-L or BRD4-S [i.e., S(a)] antibody or with Abcam EN1 antibody (Cat# ab108598). Sequencing libraries were constructed using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) following manufacturer's protocol.