Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ZFX

Cell type

Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
HEK293T
cell line
kidney cell line HEK293T
factor
ZFX ZF 1-8 FLAG
chip antibody
Sigma, F1804, lot SLBX2256

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatins were prepared using the protocol previously published (PMID: 29429977). Briefly, cells were crosslinked by 1%formedelhyde for 10 mins and lysised into nuclei. Nuclei were sonicated using BioRuptor to break into chromatin fragments. Librarys were prepared using KAPA Hyper Prep Kit (Kapa #KK8505) according to provided protocol

Sequencing Platform

instrument_model
Illumina HiSeq 3000

hg19

Number of total reads
19382288
Reads aligned (%)
89.9
Duplicates removed (%)
6.5
Number of peaks
990 (qval < 1E-05)

hg38

Number of total reads
19382288
Reads aligned (%)
91.8
Duplicates removed (%)
5.4
Number of peaks
818 (qval < 1E-05)

Base call quality data from DBCLS SRA