Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ZFX

Cell type

Cell type Class
Prostate
Cell type
22Rv1
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
22Rv1
cell line
prostate cell line 22Rv1
factor
ZFX
chip antibody
CST, L28B6, lot 1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatins were prepared using the protocol previously published (PMID: 29429977). Briefly, cells were crosslinked by 1%formedelhyde for 10 mins and lysised into nuclei. Nuclei were sonicated using BioRuptor to break into chromatin fragments. Librarys were prepared using KAPA Hyper Prep Kit (Kapa #KK8505) according to provided protocol

Sequencing Platform

instrument_model
Illumina HiSeq 3000

hg19

Number of total reads
20787100
Reads aligned (%)
87.7
Duplicates removed (%)
10.3
Number of peaks
3903 (qval < 1E-05)

hg38

Number of total reads
20787100
Reads aligned (%)
90.0
Duplicates removed (%)
8.9
Number of peaks
3881 (qval < 1E-05)

Base call quality data from DBCLS SRA