Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
HIF1A

Cell type

Cell type Class
Lung
Cell type
BEAS-2B
Primary Tissue
Lung
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
Bronchial epithelial cells
tissue
lung, bronchus
cell line
BEAS-2B
chip antibody
HIF1α (Abcam cat#ab2185)
treatment
control

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Ten million control and arsenic treated BEAS-2B cells were fixed and subjected to immunoprecipitation using ChIP-grade antibodies against Nrf2 and HIF1α. ChIP and input/control DNA were prepared for sequencing using Illumina NextSeq 500. Libraries were generated using Next Gen DNA library kit

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
41643444
Reads aligned (%)
69.4
Duplicates removed (%)
26.1
Number of peaks
5255 (qval < 1E-05)

hg38

Number of total reads
41643444
Reads aligned (%)
71.2
Duplicates removed (%)
24.6
Number of peaks
5400 (qval < 1E-05)

Base call quality data from DBCLS SRA