Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NFE2L2

Cell type

Cell type Class
Lung
Cell type
BEAS-2B
Primary Tissue
Lung
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
Bronchial epithelial cells
tissue
lung, bronchus
cell line
BEAS-2B
chip antibody
Nrf2 (Santa Cruz cat#sc-13032)
treatment
arsenic

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Ten million control and arsenic treated BEAS-2B cells were fixed and subjected to immunoprecipitation using ChIP-grade antibodies against Nrf2 and HIF1α. ChIP and input/control DNA were prepared for sequencing using Illumina NextSeq 500. Libraries were generated using Next Gen DNA library kit

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
33509851
Reads aligned (%)
69.9
Duplicates removed (%)
26.4
Number of peaks
5205 (qval < 1E-05)

hg38

Number of total reads
33509851
Reads aligned (%)
71.3
Duplicates removed (%)
25.3
Number of peaks
5245 (qval < 1E-05)

Base call quality data from DBCLS SRA