Antigen

Antigen Class

TFs and others

Antigen

GATA2

Cell type

Cell type Class

Uterus

Cell type

Endometrial stromal cells

NA

NA

Sample

source_name

Primary endometrial stromal cells

cell type

Isolated stromal cells post-hysterectomy

patient id

Patient 1

antibody

GATA2 ChIP DNA

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

7.5e8 cells (patient number = 2) were used for ChIP-Seq using the SimpleChIP Kit (Cell Signaling Technology, #9005). GATA2 antibody was purchased from Santa Cruz (#sc-9008). Next-generation sequencing libraries were prepared using the KAPA Hyper Prep Kit (KAPA Biosystems, KK8502) and KAPA Single-Indexed Adapter Kit (KAPA Biosystems, KK8710).

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

21122216

Reads aligned (%)

96.2

Duplicates removed (%)

15.1

Number of peaks

15928 (qval < 1E-05)

hg19

Number of total reads

21122216

Reads aligned (%)

95.6

Duplicates removed (%)

15.8

Number of peaks

15928 (qval < 1E-05)