Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
YY1

Cell type

Cell type Class
Blood
Cell type
T-ALL
NA
NA

Attributes by original data submitter

Sample

source_name
Human T-ALL cells
antibody
YY1 (61779, active motif)
strain
NOG mice
age
3-4 week-old
Sex
female

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were cross-linked with 1% formaldehyde for 10 min at room temperature and formaldehyde was then inactivated by the addition of 125 mM glycine for 5min. Sonicated DNA fragments with 100–300 bp were pre-cleared and then immunoprecipitated with Protein A+G Magnetic beads coupled with Anti-YY1 antibody (61779, active motif). After reverse crosslinking, immunoprecipitated DNAs and input DNAs were end-repaired and ligated adapters to the DNA fragments.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
36174891
Reads aligned (%)
82.7
Duplicates removed (%)
5.4
Number of peaks
17240 (qval < 1E-05)

hg38

Number of total reads
36174891
Reads aligned (%)
84.0
Duplicates removed (%)
4.5
Number of peaks
17401 (qval < 1E-05)

Base call quality data from DBCLS SRA