GSM4310198: H69 5TGF beta 3 SMAD3; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
SMAD3
Cell type
Cell type Class
Others
Cell type
H69
NA
NA
Attributes by original data submitter
Sample
source_name
Biliary Epithelial Cells
cell line
H69 cholangiocyte cell line
passage
P30-33
agent
TGF beta
antibody
SMAD3
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cross linked with 1% formaldehyde for 10 mins followed by quinching with glycine and washed with TBS. ChIP-seq was performed as described by Zhong et al., 2017. ChIP-seq libraries were prepared from about 1 - 10 ng ChIP and input DNA using the ThruPLEX® DNA-seq Kit V2 (Rubicon Genomics, Ann Arbor, MI). The libraries were sequenced to 51 base pairs from both ends on an Illumina HiSeq 4000 instrument in the Mayo Clinic Center for Individualized Medicine Medical Genomics Facility.