ChIP-Atlas: SRX7716743

Antigen

Cell type Class: Gonad
Cell type: Spermatocytes
MeSH Description: Male germ cells derived from SPERMATOGONIA. The euploid primary spermatocytes undergo MEIOSIS and give rise to the haploid secondary spermatocytes which in turn give rise to SPERMATIDS.

library_strategy: ChIP-Seq
library_source: GENOMIC
library_selection: ChIP
library_construction_protocol: Pachytene spermatocyte and round spermatid fractions were collected by StaPut (Bellve et al 1993). Purity was assessed under phase optics and was consistently >95% for each desired cell type. Cells were washed once in PBS and then split into two aliquots. One aliquot (for ChIP) was fixed in 1% formaldehyde then quenched with 2.5M glycine, while the second (for RNA) was left unfixed. Both fixed and unfixed aliquots were snap frozen in liquid nitrogen, then stored at -80C. ChIP was performed as previously described (Lesch et al 2013). ChIP-seq libraries were prepared using a TruSeq ChIP sample prep kit (Illumina) according to the manufacturer's instructions, except that size selection was performed after instead of before PCR amplification.