GSM1556149: input DNA undiff; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Muscle
Cell type
C2C12
Primary Tissue
Skeletal Muscle
Tissue Diagnosis
NOS
Attributes by original data submitter
Sample
source_name
Undifferentiated C2C12 cells
cell line
C2C12
cell type
Undifferentiated C2C12 cells
chip antibody
none (input)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was done with MAGnify™ Chromatin Immunoprecipitation System (life technologies, Cat#: 49-2024) following the manufacturer’s indication with some modifications. Sonication was performed using Biorupter UCD300 to obtain chromatin fragments of approximately between 100-300bp. The following antibodies were used: anti-H3K4me2 (Abcam ab7766), anti-H3K4me3 (Abcam ab8580) and anti-MyoD (Santa Cruz, sc-760). Libraries were prepared (with modifications) using NEXTflex™ ChIP-Seq Kit (Bioo Scientific, Cat#: 5143). Sequencing was done with Illumina Hiseq 2000, producing 51bp+7 single reads.