Antigen

Antigen Class

TFs and others

Antigen

SUMO2

Cell type

Cell type Class

Prostate

Cell type

VCaP

Primary Tissue

Prostate

Tissue Diagnosis

Carcinoma

Sample

source_name

VCaP_SUMO2/3_R1881

cell line

Vertebral Cancer of Prostate (VCaP)

cell line origin

bone metastasis of prostate cancer

exposed to

10 nM R1881 for 2hrs

chip antibody

Anti-SUMO-2/3 mAb

chip antibody vendor

MBL International Corporation

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were derived from sonicated nuclei and AR-, PIAS1-, FOXA1-, Pol2-, SUMO2/3- and H3K4me2-DNA complexes were isolated with 1 ug (one microgram) of antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocol, as utilized at the Functional Genomics Unit, Helsinki, Finland, BGI Tech Solutions, Shenzhen, China or at the Gene Core in EMBL, Heidelberg, Germany.

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

41948633

Reads aligned (%)

98.1

Duplicates removed (%)

2.8

Number of peaks

2920 (qval < 1E-05)

hg19

Number of total reads

41948633

Reads aligned (%)

97.4

Duplicates removed (%)

4.1

Number of peaks

2899 (qval < 1E-05)