GSM4301511: MAFF ChIP-seq Input normoxia; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Breast
Cell type
MDA-MB-231
Primary Tissue
Breast
Site of Extraction
Effusion, Pleural
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
a human breast cancer cell line
origin
mammary gland/breast; derived from metastatic site: pleural effusion
disease
adenocarcinoma
antibody or sirna
N/A
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-seq, after fixation with 37% formaldehyde, nuclei was collected by sonication (Bioruptor, Diagenode). DNA-MAFF bound was immunoprecipitated with a MAFF-specific antibody. For RNA-seq, RNA was collected using a Rneasy kit (Qiagen) For ChIP-seq, libraries were prepared using NEBNext ChIP-Seq Library Prep Master Mix set for Illumina (NEB #E6240) and NEBNext Multiplex Oligos for Illumina (NEB #E7730S). For RNA-seq, libraries were prepared using Illumina's TruSeq RNA library prep kit v2. ChIP seq and RNA seq