Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Epidermis
Cell type
451Lu
Primary Tissue
Skin
Tissue Diagnosis
Melanoma

Attributes by original data submitter

Sample

source_name
451Lu
cell line
451Lu
cell type
melanoma
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Twenty million cells were used for the ChIP and precipitated using 5 micrograms of antibody (cell signaling, 4356) against human HSF1 Libraries were generated as described before, including end-repair, A-tailing, adapter (Illumina Truseq system) ligation and PCR amplification.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
34413537
Reads aligned (%)
98.7
Duplicates removed (%)
4.5
Number of peaks
696 (qval < 1E-05)

hg19

Number of total reads
34413537
Reads aligned (%)
98.1
Duplicates removed (%)
5.8
Number of peaks
775 (qval < 1E-05)

Base call quality data from DBCLS SRA