GSM1553235: ChIP-Seq analysis of HSF1 in 451Lu melanoma untreated cells replicate1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
HSF1
Cell type
Cell type Class
Epidermis
Cell type
451Lu
Primary Tissue
Skin
Tissue Diagnosis
Melanoma
Attributes by original data submitter
Sample
source_name
451Lu
cell line
451Lu
cell type
melanoma
chip antibody
HSF1 (Cell Signaling, catalog# 4356, lot# 2)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Twenty million cells were used for the ChIP and precipitated using 5 micrograms of antibody (cell signaling, 4356) against human HSF1 Libraries were generated as described before, including end-repair, A-tailing, adapter (Illumina Truseq system) ligation and PCR amplification.