Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
HDAC2

Cell type

Cell type Class
Neural
Cell type
U-87 MG
Primary Tissue
Brain
Tissue Diagnosis
Glioblastoma

Attributes by original data submitter

Sample

source_name
U87 glioblastoma cell line
cell type
Glioblastoma cell line
treatment
DMSO
chip antibody
HDAC2 (Cell Signalling, 57156)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was extracted using the SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005 from Cell Signalling. next generation sequencing (NGS) from Genewiz, New Jersey

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
35374709
Reads aligned (%)
54.3
Duplicates removed (%)
11.7
Number of peaks
442 (qval < 1E-05)

hg38

Number of total reads
35374709
Reads aligned (%)
55.8
Duplicates removed (%)
10.6
Number of peaks
734 (qval < 1E-05)

Base call quality data from DBCLS SRA