Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
Kasumi-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
Kasumi-1_input
cell line
Kasumi-1
cell type
myeloblast
disease
acute myeloblastic leukemia
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from crosslinked and sonicated cells and JMJD1C-DNA complexes were isolated with antibody. The antibody was home-made and hasn't been published yet. The antigen used for this antibody generation is the N-terminus of JMJD1C protein. Libraries were prepared according to Illumina's instructions

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
45773148
Reads aligned (%)
96.8
Duplicates removed (%)
6.4
Number of peaks
1498 (qval < 1E-05)

hg19

Number of total reads
45773148
Reads aligned (%)
95.7
Duplicates removed (%)
8.1
Number of peaks
1361 (qval < 1E-05)

Base call quality data from DBCLS SRA