GSM4283790: input.bam.bigwig; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Breast
Cell type
BT-474
Primary Tissue
Breast
Site of Extraction
Soft Tissue
Tissue Diagnosis
Adenocarcinoma Ductal
Attributes by original data submitter
Sample
source_name
BT-474 human breast cancer cell line treated with vehicle for 90 minutes
tissue
Breast tumor cell line
chip antibody
input DNA
chip-antibody
input DNA as control ChIP-seq
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
After 90min following media renewal, nuclei were isolated and subjected to chromatin immunoprecipitation. Following ERRgamma ChIP, genomic DNA was purified with QIAGEN DNA extraction kit. Libraries were performed at the Génome Québec Innovation Centre (McGill University) using standard protocol for HiSeq - Illumina Single reads 50bp sequencing lane