Antigen

Antigen Class

TFs and others

Antigen

ESRRG

Cell type

Cell type Class

Breast

Cell type

BT-474

Primary Tissue

Breast

Site of Extraction

Soft Tissue

Tissue Diagnosis

Adenocarcinoma Ductal

Sample

source_name

BT-474 human breast cancer cell line treated with vehicle for 90 minutes

tissue

Breast tumor cell line

chip antibody

ERRg antibody (custom antibody, Dr. Ron Evans lab)

chip-antibody

genomic DNA enriched following ERRgamma chromatin immunoprecipitation

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

After 90min following media renewal, nuclei were isolated and subjected to chromatin immunoprecipitation. Following ERRgamma ChIP, genomic DNA was purified with QIAGEN DNA extraction kit. Libraries were performed at the Génome Québec Innovation Centre (McGill University) using standard protocol for HiSeq - Illumina Single reads 50bp sequencing lane

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

89638210

Reads aligned (%)

92.6

Duplicates removed (%)

9.5

Number of peaks

7823 (qval < 1E-05)

hg19

Number of total reads

89638210

Reads aligned (%)

91.7

Duplicates removed (%)

11.3

Number of peaks

7556 (qval < 1E-05)