Antigen

Antigen Class

TFs and others

Antigen

elav

Cell type

Cell type Class

Embryo

Cell type

Embryos

NA

NA

Sample

source_name

whole embryos

hours after fertilization

10-12

chip antibody

ELAV

chip antibody vendor

Developmental Studies Hybridoma Bank (DSHB)

chip catalog number

mouse anti-ELAV-9F8A9

chip catalog number

rat anti-ELAV-7E8A10

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Whole embryo nuclei extracts were sonicated and ChIP assays were done using a mix of two anti-ELAV antibodies raised against the entire ELAV protein: mouse anti-ELAV-9F8A9 (DSHB) and rat anti-ELAV-7E8A10 (DSHB). ChIP-seq libraries were constructed with the NEBNext ChIP-seq Library Prep Master Mix Set for Illumina using NEBNext Multiplex Oligos.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

dm6

Number of total reads

19472584

Reads aligned (%)

83.6

Duplicates removed (%)

47.0

Number of peaks

2396 (qval < 1E-05)

dm3

Number of total reads

19472584

Reads aligned (%)

90.0

Duplicates removed (%)

48.3

Number of peaks

2010 (qval < 1E-05)