Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
elav

Cell type

Cell type Class
Embryo
Cell type
Embryos
NA
NA

Attributes by original data submitter

Sample

source_name
whole embryos
hours after fertilization
10-12
chip antibody
ELAV
chip antibody vendor
Developmental Studies Hybridoma Bank (DSHB)
chip catalog number
mouse anti-ELAV-9F8A9
chip catalog number
rat anti-ELAV-7E8A10

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Whole embryo nuclei extracts were sonicated and ChIP assays were done using a mix of two anti-ELAV antibodies raised against the entire ELAV protein: mouse anti-ELAV-9F8A9 (DSHB) and rat anti-ELAV-7E8A10 (DSHB). ChIP-seq libraries were constructed with the NEBNext ChIP-seq Library Prep Master Mix Set for Illumina using NEBNext Multiplex Oligos.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm3

Number of total reads
22379909
Reads aligned (%)
53.3
Duplicates removed (%)
37.9
Number of peaks
3455 (qval < 1E-05)

dm6

Number of total reads
22379909
Reads aligned (%)
50.2
Duplicates removed (%)
38.3
Number of peaks
3844 (qval < 1E-05)

Base call quality data from DBCLS SRA