Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
X18.1.1
NA
NA

Attributes by original data submitter

Sample

source_name
X18.1.1 AML cell line, empty vector, H3K27Ac ChIP
cell line
X18.1.1
cell type
AML cell line
transfection
empty vector
chip antibody
H3K27Ac (Abcam ab4729)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Illumina HiSeq 2000

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
28968902
Reads aligned (%)
91.4
Duplicates removed (%)
24.5
Number of peaks
29552 (qval < 1E-05)

mm9

Number of total reads
28968902
Reads aligned (%)
91.3
Duplicates removed (%)
24.5
Number of peaks
29556 (qval < 1E-05)

Base call quality data from DBCLS SRA