Curated Sample Data


Genome
mm9
Antigen Class
Input control
Antigen
Input control
Cell type Class
Blood
Cell type
Hematopoietic Stem Cells

Cell type information


MeSH Description
Progenitor cells from which all blood cells derived. They are found primarily in the bone marrow and also in small numbers in the peripheral blood.

Attributes by Original Data Submitter


source_name
Hematopoietic stem cells (HSC)
cell type
Murine bone marrow derived primary HSCs
cell surface marker
SP-KSL-CD150+
strain
C57BL/6
chip antibody
NOT APPLICABLE

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Genomic DNA were extracted from the cells and fragmented to 200-500 bp via biorupter. Followed by immunoprecipitation with specific antibody, ChIPed DNA were successfully made library using ThruPLEX preparation kit without extra amplification (Rubicon, Ann Arbor, MI)

Platform Information


instrument_model
Illumina HiSeq 2000

External Database Query

Logs in read processing pipeline


Number of total reads
38029789
Reads aligned (%)
93.3
Duplicates removed (%)
14.0
Number of peaks
214 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA