Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Neural
Cell type
Cortical neuron
NA
NA

Attributes by original data submitter

Sample

source_name
mouse primary cortical neuron
antibody
Anti- Pol II Santa Cruz Biotechnology (Cat. No. sc-899X)
passages
LSD1n WT DIV10
cell type
mouse primary cortical neuron
treatment
KCl
time
1 hr

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Sonicated nuclei were precleared with Protein G magnetic beads and antibody chromatin complexes were isolated, and eluted DNA was reverse cross-linked overnight and DNA was purified using Qiagen DNA purification columns using Manufacturer's recommended protocol. Libraries were prepared using Illumina's recommended protocol

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
27948015
Reads aligned (%)
94.6
Duplicates removed (%)
6.4
Number of peaks
11502 (qval < 1E-05)

mm9

Number of total reads
27948015
Reads aligned (%)
94.5
Duplicates removed (%)
6.5
Number of peaks
11540 (qval < 1E-05)

Base call quality data from DBCLS SRA