Anti- Pol II Santa Cruz Biotechnology (Cat. No. sc-899X)
passages
LSD1n WT DIV10
cell type
mouse primary cortical neuron
treatment
KCl
time
0 hr
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Sonicated nuclei were precleared with Protein G magnetic beads and antibody chromatin complexes were isolated, and eluted DNA was reverse cross-linked overnight and DNA was purified using Qiagen DNA purification columns using Manufacturer's recommended protocol. Libraries were prepared using Illumina's recommended protocol