Antigen

Antigen Class

TFs and others

Antigen

FOXO1

Cell type

Cell type Class

Bone

Cell type

Chondrocytes

MeSH Description

Polymorphic cells that form cartilage.

Sample

source_name

FoxO1_ChIP-seq

donor status

total knee replacement surgery patient

cell type

primary chondrocytes

chip antibody

FoxO1

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

FoxO1-DNA complexes were isolated with antibody. For library preparation, 10 ng of each sample was prepared using the NEB Ultra DNA Library Prep Kit for Illumina following manufacturer's instructions.

Sequencing Platform

instrument_model

NextSeq 500

hg19

Number of total reads

18540572

Reads aligned (%)

83.0

Duplicates removed (%)

14.3

Number of peaks

984 (qval < 1E-05)

hg38

Number of total reads

18540572

Reads aligned (%)

85.6

Duplicates removed (%)

12.6

Number of peaks

910 (qval < 1E-05)