Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
FOXP1

Cell type

Cell type Class
Blood
Cell type
OCI-LY8
NA
NA

Attributes by original data submitter

Sample

source_name
OCI-Ly8 GCB-DLBCL B cells
cell type
Germinal Center like Diffuse Large B Cell Lymphoma cell line
chip antibody
Anti-FOXP1 polyclonal (Gift from Ed Morrisey lab)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed by cross-linking proteins to DNA using 1% formaldehyde solution. Immunoprecipitated DNA was sequenced using Illumina sequencers. Pull-downed DNA was applied for generation of sequencing libraries using the NEB ChIP-seq library prep. kit (E6200L ) following by manufacturer’s instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
48919138
Reads aligned (%)
18.3
Duplicates removed (%)
20.1
Number of peaks
4221 (qval < 1E-05)

hg38

Number of total reads
48919138
Reads aligned (%)
18.7
Duplicates removed (%)
19.2
Number of peaks
4105 (qval < 1E-05)

Base call quality data from DBCLS SRA