Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
FOXP1

Cell type

Cell type Class
Blood
Cell type
HT
Primary Tissue
Blood
Site of Extraction
Ascites
Tissue Diagnosis
Lymphoma Diffuse Mixed

Attributes by original data submitter

Sample

source_name
HT GCG-DLBCL B cells
cell type
Germinal Center like Diffuse Large B Cell Lymphoma cell line
chip antibody
Anti-FOXP1 polyclonal (Gift from Ed Morrisey lab)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed by cross-linking proteins to DNA using 1% formaldehyde solution. Immunoprecipitated DNA was sequenced using Illumina sequencers. Pull-downed DNA was applied for generation of sequencing libraries using the NEB ChIP-seq library prep. kit (E6200L ) following by manufacturer’s instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
55068297
Reads aligned (%)
20.0
Duplicates removed (%)
36.9
Number of peaks
7415 (qval < 1E-05)

hg38

Number of total reads
55068297
Reads aligned (%)
20.5
Duplicates removed (%)
35.9
Number of peaks
7297 (qval < 1E-05)

Base call quality data from DBCLS SRA