Antigen

Antigen Class

TFs and others

Antigen

FOXP1

Cell type

Cell type Class

Blood

Cell type

HT

Primary Tissue

Blood

Site of Extraction

Ascites

Tissue Diagnosis

Lymphoma Diffuse Mixed

Sample

source_name

HT GCG-DLBCL B cells

cell type

Germinal Center like Diffuse Large B Cell Lymphoma cell line

chip antibody

Anti-FOXP1 polyclonal (Gift from Ed Morrisey lab)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP was performed by cross-linking proteins to DNA using 1% formaldehyde solution. Immunoprecipitated DNA was sequenced using Illumina sequencers. Pull-downed DNA was applied for generation of sequencing libraries using the NEB ChIP-seq library prep. kit (E6200L ) following by manufacturer’s instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

55068297

Reads aligned (%)

31.9

Duplicates removed (%)

35.9

Number of peaks

7297 (qval < 1E-05)

hg19

Number of total reads

55068297

Reads aligned (%)

31.6

Duplicates removed (%)

36.9

Number of peaks

7415 (qval < 1E-05)