Antigen

Antigen Class

TFs and others

Antigen

ATF4

Cell type

Cell type Class

Blood

Cell type

HUDEP-2

NA

NA

Sample

source_name

HUDEP2-ATF4

cell line

HUDEP2

tissue

Immortalized Human Erythroid Progenitor

cell type

Erythroid cells

differentiation

Undifferentiated

chip antibody

ATF4 (Cell Signaling Technology, 11815S, lot 4)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were crosslinked with 1% formaldehyde for 10 minutes at room temperature. Fixed cells were lysised in 1mL Cell Lysis Buffer (10mM Tris-HCl pH 8, 10mM NaCl, 0.2% NP-40/Igepal). Nuclei were collected and lysised in 1mL Nuclear Lysis Buffer (50mM Tris-HCl pH 8, 10mM EDTA pH 8, 1% SDS). Chromatin was sonicated at 4 degrees C (Epishear, Active Motif). Chromatin was incubated with antibody and then decrosslink at 65C overnight. Libraries were prepared using TruSeq ChIP-seq Sample Preparation Kit (Part# IP-202-1012) according to Illumina's instructions.

Sequencing Platform

instrument_model

NextSeq 500

hg19

Number of total reads

34607618

Reads aligned (%)

78.3

Duplicates removed (%)

17.7

Number of peaks

2351 (qval < 1E-05)

hg38

Number of total reads

34607618

Reads aligned (%)

81.0

Duplicates removed (%)

15.6

Number of peaks

2737 (qval < 1E-05)