Antigen

Antigen Class

TFs and others

Antigen

BRD4

Cell type

Cell type Class

Muscle

Cell type

RH-4

NA

NA

Sample

source_name

RH4

antibody

BRD4, Bethyl Lab (A301-985A100)

enrichment target

BRD4

condition

Epigenetic regulator

shRNA/target function

shRNA-scramble

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP samples harvested after 1% formaldehyde fixation for 12 minutes, sonicated, processed and ChIP-enriched via standard protocols (Active Motif ChIP-IT HS kit). ChIP enriched DNA was treated with Proteinase K at 55 ˚C for 30 minutes followed by reversal of crosslinks by heating to 80 ˚C for 2 hours, and DNA purification by column (MiniElute PCR purification kit, Qiagen). DNA was extracted from ChIP-seq samples prepared following the Active Motif ChIP-IT High Sensitivity kit protocol. Standard Illumina barcodes were introduced during library preparation with the Illumina TruSeq ChIP Sample Prep Kit 48 Samples - Set A Cat# IP-202-1012

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

26188819

Reads aligned (%)

95.8

Duplicates removed (%)

11.1

Number of peaks

6371 (qval < 1E-05)

hg19

Number of total reads

26188819

Reads aligned (%)

95.0

Duplicates removed (%)

13.5

Number of peaks

6446 (qval < 1E-05)