Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
AR

Cell type

Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
ChIP-seq with AR, 1h treatment with 100 nM DHT
cell line
LNCaP
chip antibody
AR (Santa Cruz; sc-816)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation experiments were done as previously described (Garcia-Bassets et al., 2007), global run-on sequencing (GRO-seq) was performed as detailed (Wang et al., 2011) and precision nuclear run-on sequencing of transcription initiation sites (PRO-cap) was performed as described (Kwak et al., 2013).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
120095509
Reads aligned (%)
53.1
Duplicates removed (%)
24.6
Number of peaks
5754 (qval < 1E-05)

hg38

Number of total reads
120095509
Reads aligned (%)
54.3
Duplicates removed (%)
23.4
Number of peaks
6546 (qval < 1E-05)

Base call quality data from DBCLS SRA