Curated Sample Data


Genome
ce10
Antigen Class
Histone
Antigen
H3K36me3
Cell type Class
Larvae
Cell type
L1

Cell type information


NA
NA

Attributes by Original Data Submitter


source_name
L1 larvae
strain
spr-5 (by101)(I); met-2 (n4256)(III)
tissue
whole animal
developmental stage
L1 larvae
chip antibody
H3K36me3

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
600 starved L1 larvae born at room temperature (21° C - 22° C) overnight in M9 Buffer were collected, frozen in liquid nitrogen, and stored at -80° C prior to homogenization. Frozen pellets were disrupted by a glass Dounce homogenizer, fixed with formaldehyde (1% final concentration), and quenched with glycine. ChIP samples were processed with a Chromatin Immunoprecipitation Assay Kit (Millipore), according to manufacturer's instructions. Samples were sonicated using a Diagenode Bioruptor UCD-200 at 4 °C on the “high” setting for a total of 30 min with a cycle of 45 secs on and 15 secs off. A total of 12.5 μL (5 ug) H3K36me3 antibody (cat. 61021; Active motif) was used for immunoprecipitation. The Genomic Services Laboratory (GSL) (HudsonAlpha, Huntsville, Alabama) performed library preparation and sequencing (Illumina HiSeq v4, 50bp single-end reads). Libraries were prepared by HAIB-GSL (for HiSeq).

Platform Information


instrument_model
Illumina HiSeq 2500

External Database Query

Logs in read processing pipeline


Number of total reads
29603116
Reads aligned (%)
55.5
Duplicates removed (%)
45.1
Number of peaks
806 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA