Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
KLF5

Cell type

Cell type Class
Digestive tract
Cell type
TE-5
Primary Tissue
Esophagus
Tissue Diagnosis
Carcinoma Squamous Cell

Attributes by original data submitter

Sample

source_name
TE5_KLF5 ChIP-Seq
cell type
Esophageal Squamous cell carcinomas cell
antibody
KLF5
cell line
TE5

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Total RNA was extracted by RNeasy Mini kit (QIAGEN) according to the manufacturer's instructions. Standard Illumina library preparation protocol

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

hg19

Number of total reads
23775257
Reads aligned (%)
157.8
Duplicates removed (%)
4.5
Number of peaks
485 (qval < 1E-05)

hg38

Number of total reads
23775257
Reads aligned (%)
159.6
Duplicates removed (%)
4.4
Number of peaks
816 (qval < 1E-05)

Base call quality data from DBCLS SRA