Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Digestive tract
Cell type
KYSE-150
Primary Tissue
Esophagus
Tissue Diagnosis
Carcinoma Squamous Cell

Attributes by original data submitter

Sample

source_name
KYSE150_input ChIP-Seq
cell type
Esophageal Squamous cell carcinomas cell
cell line
KYSE150

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Total RNA was extracted by RNeasy Mini kit (QIAGEN) according to the manufacturer's instructions. Standard Illumina library preparation protocol

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

hg38

Number of total reads
12373355
Reads aligned (%)
80.3
Duplicates removed (%)
4.9
Number of peaks
347 (qval < 1E-05)

hg19

Number of total reads
12373355
Reads aligned (%)
79.5
Duplicates removed (%)
5.0
Number of peaks
174 (qval < 1E-05)

Base call quality data from DBCLS SRA