Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SREBF1

Cell type

Cell type Class
Digestive tract
Cell type
KYSE-150
Primary Tissue
Esophagus
Tissue Diagnosis
Carcinoma Squamous Cell

Attributes by original data submitter

Sample

source_name
KYSE150_SREBF1 ChIP-Seq
cell type
Esophageal Squamous cell carcinomas cell
antibody
Anti-SREBF1
cell line
KYSE150

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Total RNA was extracted by RNeasy Mini kit (QIAGEN) according to the manufacturer's instructions. Standard Illumina library preparation protocol

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

hg19

Number of total reads
23225628
Reads aligned (%)
133.5
Duplicates removed (%)
5.5
Number of peaks
2047 (qval < 1E-05)

hg38

Number of total reads
23225628
Reads aligned (%)
134.9
Duplicates removed (%)
5.4
Number of peaks
2355 (qval < 1E-05)

Base call quality data from DBCLS SRA