Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Lpt

Cell type

Cell type Class
Larvae
Cell type
3rd instar
NA
NA

Attributes by original data submitter

Sample

source_name
wandering third instar larvae
genotype
Y cn bw sp
antibody
anti-Cmi

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was crosslinked at RT in 1.8% formaldehyde and homogenized for chromatin extraction. Cmi antiserum was used for immunoprecipitation. Input and immunoprecipitated DNA were prepared for sequencing using the Illumina TruSeq ChIP Sample Preparation Kit per the manufacturer's instructions. Library construction was performed by the University of Chicago IGSB Sequencing Core.

Sequencing Platform

instrument_model
Illumina Genome Analyzer

dm3

Number of total reads
12211779
Reads aligned (%)
76.7
Duplicates removed (%)
13.9
Number of peaks
643 (qval < 1E-05)

dm6

Number of total reads
12211779
Reads aligned (%)
75.6
Duplicates removed (%)
14.9
Number of peaks
290 (qval < 1E-05)

Base call quality data from DBCLS SRA